Abstract We investigated the feasibility of producing bioethanol from mixed softwood pretreated with the ionic liquid 1-butyl-3-methylimidazolium acetate ([Bmim]Ac). The optimal pretreatment conditions were determined by response surface methodology to be 100°C for 15 h, and the fermentable sugar yield was estimated to be 92.5%. Efficient pretreatment of softwood was maintained even after reutilizing [Bmim]Ac up to four times. Through the enzymatic saccharification and subseq

Highlights Adding water to IL reduces the viscosity and facilitates high biomass loading. Acidified [Bmim]Cl pretreatment efficiently removed hemicellulose. Cellulose conversion of pretreated biomass was 93.56–99.81%. Fermentation produced 21.86–29.56 g/L ethanol and ethanol yield was 0.49–0.51 g/g. Produced bioethanol was concentrated up to 79.8% by consecutive pervaporation. Abstract Acidified [Bmim]Cl solution showed a great efficacy for the pretreatment of softwood, hardw

Abstract Spent coffee ground has attracted increasing attentions since it contains many useful components such as polysaccharides,...

Abstract Currently, there is worldwide interest in the technological use of agro-industrial residues as a renewable source of food and biofuels. Lignocellulosic materials (LCMs) are a rich source of cellulose and hemicellulose. Hemicellulose is rich in xylan, a polysaccharide used to develop technology for producing alcohol, xylose, xylitol and xylo-oligosaccharides (XOSs). The XOSs are unusual oligosaccharides whose main constituent is xylose linked by β 1–4 bonds. The XOS a

Soluble sugars not only serve as nutrients, but also act as signals for plant growth and development, but how sugar signals are perceived and translated into physiological responses in plants remains unclear. We manipulated sugar levels in transgenic plants by overexpressing sucrose synthase (SuSy), which is a key enzyme believed to have reversible sucrose synthesis and sucrose degradation functions. The ectopically expressed SuSy protein exhibited sucrose-degrading activity,

Highlights Enzymatic treatment was highly effective to release gallic acid from the flowers. Rose and camellia flowers are abundant sources of natural gallic acid. Protocatechuic acid achieved the highest levels using aqueous ethanol at 180 °C. Enzyme-assisted process resulted in the maximum sugar yields (380–596 mg/g). Co-production of phenolic compounds and sugars in flowers is a potential strategy. Introduction Edible flowers have been used to improve the appearance, taste

Abstract Cellulosic bioethanol production has been fraught with challenges, including fluctuations in feedstock supply, handling costs, pretreatment, enzymes, and other logistical problems. Most studies of lignocellulosic ethanol production have focused on a single type of biomass; however, full utilization of various lignocellulosic biomass sources might enhance bioethanol production and the economic feasibility of the biorefinery. The goal of this study was to evaluate the

Abstract Here, we report an increase in biomass yield and saccharification in transgenic tobacco plants ( Nicotiana tabacum L .) overexpressing thermostable β-glucosidase from Thermotoga maritima , BglB , targeted to the chloroplasts and vacuoles. The transgenic tobacco plants showed phenotypic characteristics that were significantly different from those of the wild-type plants. The biomass yield and life cycle (from germination to flowering and harvest) of the transgenic to

Phenolic compounds occur in plants as secondary metabolites with potent biological activity to control pathogens. Tomato is the world’s second most cultivated vegetable but suffers attack by Fusarium oxysporum , causing Fusarium wilt disease that severely impacts crop yield and quality. This study focused on four phenolic-rich plant extracts and their mixtures to control F. oxysporum in vitro and in tomatoes. The highest phenolic sources were Eucalyptus camaldulensis , Chro